Enzymes and Inhibitors
Selleck Chemical LLC D-Luciferin
D-Luciferin is a popular bioluminescent substrate of luciferase in the presence of ATP used in luciferase-based bioluminescence imaging and cell-based high-throughput screening applications In an immunocompetent mouse model of ovarian cancer the use of D-luciferin substrate and firefly luciferase preserves tumour-host immune interactions since bioluminescent imaging is a more sensitive indication of tumour growth than weight gain
Surmodics Inc TMB Extended Range HRP Microwell Substrate, 1L
The BioFX™ one component 3,3’,5,5’-tetramethylbenzidine (TMB) substrate is supplied in a ready-to-use solution. Peroxidase reacts with the TMB substrate solution to produce a soluble blue reaction product that can be read at 370 nm or 620-650 nm for ELISA applications. Stop solutions can be added to prevent further color development and reading the absorbance values at the desired wavelengths.
Medchemexpress LLC Her2/cd340 protein, rhesus macaque (HEK293, His) | >95.0% | 10 UG
Recombinant HER2/CD340 protein derived from rhesus macaque, expressed in HEK293 cells and supplied with a C-terminal His tag. Purified (>95% purity) and formulated for use in biochemical assays, antibody development, binding studies, and assay controls.
- Recombinant extracellular domain of HER2 protein.
- Derived from rhesus macaque.
- Expressed in HEK293 cells.
- C-terminal His tag for purification and detection.
- Purity greater than 95% by reducing SDS-PAGE.
- Available in microgram-scale quantities, including 10 μg.
- Store at -20°C; after reconstitution stable at 4°C for 1 week.
- Suitable for biochemical assays, antibody development, and binding studies.
Medchemexpress LLC D-luciferin (sodium) | 103404-75-7 | 99.9% | 302.30 | C11H7N2NaO3S2 | 500MG
D-Luciferin sodium is the sodium salt of D-luciferin, the natural substrate for firefly luciferase used to generate bioluminescent signals in reporter assays and imaging. Supplied as a light yellow solid with high purity, it is suitable for in vitro and in vivo bioluminescence applications.
- Natural substrate for firefly luciferase.
- Produces bioluminescent signal for reporter assays and imaging.
- High purity (99.9%).
- Molecular weight 302.30.
- Formula C11H7N2NaO3S2.
- Available in multiple pack sizes, including 500 MG.
- Solid, light yellow to yellow appearance.
- Store sealed, away from moisture and light; in solvent store at -80°C (6 months) or -20°C (1 month).
Biotium Coelenterazine (ALSO KNOWN AS NATIVE COE
Coelenterazines are luminescent enzyme substrates for apoaequorin and Renilla luciferase. Apoaequorin is used as a calcium indicator whereas Renilla luciferase is commonly used as a reporter of transcription regulation. Coelenterazine binds apoaequorin to form an aequorin complex which emits light upon calcium binding, and can measure a broad range of calcium concentrations from ~0.1 uM to >100 uM. Renilla luciferase is widely used as a reporter protein and as a donor in bioluminescence resonance energy transfer (BRET) to study protein-protein interactions. Coelenterazines can also be used for chemiluminescent detection of superoxide anion and peroxynitrite in cells or tissues.Coelenterazine (native): Yellow solid soluble in MeOH or EtOH; Store at -20°C and protect from light; C26H21N3O3; MW: 423; [55779-48-1]. See also other Coelentrazine analogs and water-soluble Aquaphile™ Coelenterazines (native and analog h) for in vivo use.
Revvity Health Sciences Inc LANCE Ultra Protein A Ulight Peptide Substrate, 1 nmole
LANCE Ultra Protein A Ulight Peptide Substrate, 1 nmole
Revvity Health Sciences Inc LANCE Ultra TK Ulight Peptide Substrate, 0.5 nmole
LANCE Ultra TK Ulight Peptide Substrate, 0.5 nmole
![Alkali Scientific D Luciferin Free Acid [4, 5 Dihydro 2 (6 hydroxy 2 benzothiazoyl) 4 thiazole carboxylic acid], 50 Mg](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660984.jpg-250.jpg)
Alkali Scientific D Luciferin Free Acid [4, 5 Dihydro 2 (6 hydroxy 2 benzothiazoyl) 4 thiazole carboxylic acid], 50 Mg
D-Luciferin Free Acid is a bioluminescence substrate used for high-sensitivity detection in biochemical assays. This reagent contains D-Luciferin, a substrate that is oxidized by the enzyme luciferase, resulting in light emission that can be measured for various applications. The free acid form is commonly used in bioluminescence assays for detecting ATP or monitoring cellular activity in research and clinical settings. This 50mg product is ideal for use in laboratories requiring high sensitivity for low-light detection, providing reliable and accurate results in a variety of biological assays and experimental setups.
Echelon Biosciences Research Labs FS-3 (LysoPLD / Autotaxin substrate) 100 ug
Echelons fluorogenic Autotaxin (ATX) substrate FS-3 (L-2000) is a doubly labeled analog of LPC wherin the fluorophore is quenched through intramolecular energy transfer. Hydrolysis of the substrate produces an increase in fluorescence. This item is non returnable
Enzo Life Sciences TACE substrate II (fluorogenic) (100µg)
Sensitive fluorogenic substrate for TACE (TNFα converting enzyme, ADAM17). Also cleaved by ADAM10 and likely other ADAMs and MMPs. The kcat/Km of TACE for this substrate is 7-fold higher than for the same peptide with the Mca-Dpa fluorophore-quencher pair. FAM fluorescence is quenched by the TAMRA group until cleavage separates them. Ex.: 485nm, Em.: 535nm, although the following Ex/Em can also be used: 490,494/515,520nm. This substrate is useful for inhibitor screening, kinetic analysis, and cellular activity assay. Alternative name: ADAM17 substrate 2 (fluorogenic). Purity: ≥95% (HPLC). Formulation: Lyophilized. Solubility: Soluble in DMSO. Long Term Storage: -20°C.